Enhanced Determination of Streptococcus pneumoniae Serotypes Associated with Invasive Disease in Laos by Using a Real-Time Polymerase Chain Reaction Serotyping Assay with Cerebrospinal Fluid

Title
Enhanced Determination of Streptococcus pneumoniae Serotypes Associated with Invasive Disease in Laos by Using a Real-Time Polymerase Chain Reaction Serotyping Assay with Cerebrospinal Fluid
Authors
Blacksell, SD; Jenjaroen, K; Phetsouvanh, R; Tanganuchitcharnchai, A; Phouminh, P; Phongmany, S; Day, NPJ; Newton, PN
Keywords
PNEUMOCOCCAL CONJUGATE VACCINE; PLACEBO-CONTROLLED-TRIAL; MULTIPLEX PCR; ANTIMICROBIAL SUSCEPTIBILITY; QUELLUNG REACTION; DEVELOPING-WORLD; DOUBLE-BLIND; CHILDREN; SURVEILLANCE; 6C
Issue Date
2010
Publisher
AM J TROP MED HYG
Citation
Am. J. Trop. Med. Hyg.;SEP;2010;83;3
Abstract
A prospective hospital-based study was undertaken to define the incidence of invasive pneumococcal disease (IPD) and circulating serotypes in Laos Of 10.799 patients with hemocultures and 353 patients with cerebrospinal fluid samples. 0 21% and 5 4%, respectively, were positive for Streptococcus pneumoniae. giving a total of 35 IPD patients We developed a real-time polymerase chain reaction to detect serotypes represented in the 13-valent pneumococcal vaccine A blinded evaluation comparing serotype as defined by the Quellung reaction versus the polymerase chain reaction demonstrated 100% concordance The most frequent serotype (n = 33 patients) was 1 (n = 6), followed by serotypes 5, 6A/B/C, 14, and 23F. Serotypes represented in the 7-valent polysaccharide-protein conjugate vaccine (PCV-7) infected 39% of patients with 73% coverage for the PCV-10 and PCV-13 vaccines. Although the sample size is small, these data suggest that the PCV-7 vaccine may have relatively low efficacy in Laos Further studies are urgently needed to guide pneumococcal vaccine policy in Laos
URI
http://hdl.handle.net/11267/2334
ISSN
0002-9637
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5.Mahosot Hospital > Journal articles
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