Accuracy of Rapid IgM-Based Immunochromatographic and Immunoblot Assays for Diagnosis of Acute Scrub Typhus and Murine Typhus Infections in Laos

Title
Accuracy of Rapid IgM-Based Immunochromatographic and Immunoblot Assays for Diagnosis of Acute Scrub Typhus and Murine Typhus Infections in Laos
Authors
Blacksell, SD; Jenjaroen, K; Phetsouvanh, R; Wuthiekanun, V; Day, NPJ; Newton, PN; Ching, WM
Keywords
ORIENTIA-TSUTSUGAMUSHI; RICKETTSIA-TSUTSUGAMUSHI; ANTIBODY; FEVER; THAILAND
Issue Date
2010
Publisher
AM J TROP MED HYG
Citation
Am. J. Trop. Med. Hyg.;AUG;2010;83;2
Abstract
We studied the diagnostic accuracy of a rapid immunochromatographic test (ICT) for detection of IgM against scrub typhus (ST ICT) and an immunoblot test for the detection of IgM against murine typhus (MT IBT) by using admission serum samples from 1,030 febrile patients in Laos. Sensitivity and specificity for the Si ICT determined by using the diagnostic criteria of a tour-fold increase in IgM against Orientia tsutsugamusht between paned samples were 23.8% (95% confidence interval [CI] = 15 9-33 3%) and 86 2% (95% CI = 84 1-88.6%), respectively Sensitivity and specificity for the ST ICT determined by using an admission IgM titer >= 1 400 were 39.1% (95% CI = 34 1-44 2%) and 99 5% (95% CI = 98 7-99 9%), respectively Sensitivity and specificity for the MT IBT determined by using the criteria of a four-fold Increase in le,M against Rickettsia typht between paired serum samples were 61 2% (95% CI = 53 7-68 3%) and 86 5% (95% CI = 84 1-88 8%). respectively Sensitivity and specificity for the MT IBT determined by using an admission IgM titer >= 1 400 were 54 6% (95% CI = 49 1-60 0%) and 94.1% (95% CI = 92 0-95 7%), respectively Both assays had relatively good specificity but low sensitivity and thus have limited utility for admission diagnosis
URI
http://hdl.handle.net/11267/2335
ISSN
0002-9637
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5.Mahosot Hospital > Journal articles
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