SYBR Green Real-Time PCR for the Detection of All Enterovirus-A71 Genogroups

Title
SYBR Green Real-Time PCR for the Detection of All Enterovirus-A71 Genogroups
Authors
Dubot-Peres, A; Tan, CYQ; de Chesse, R; Sibounheuang, B; Vongsouvath, M; Phommasone, K; Bessaud, M; Gazin, C; Thirion, L; Phetsouvanh, R; Newton, PN; de Lamballerie, X
Keywords
ACUTE FLACCID PARALYSIS; MOUTH-DISEASE; RT-PCR; CLINICAL SPECIMENS; 71 INFECTION; MOLECULAR EPIDEMIOLOGY; HAND; FOOT; OUTBREAK; TAIWAN
Issue Date
2014
Publisher
PLOS ONE
Citation
PLoS One;20-Mar;2014;9;3
Abstract
Enterovirus A71 (EV-A71) has recently become an important public health threat, especially in South-East Asia, where it has caused massive outbreaks of Hand, Foot and Mouth disease every year, resulting in significant mortality. Rapid detection of EV-A71 early in outbreaks would facilitate implementation of prevention and control measures to limit spread. Real-time RTPCR is the technique of choice for the rapid diagnosis of EV-A71 infection and several systems have been developed to detect circulating strains. Although eight genogroups have been described globally, none of these PCR techniques detect all eight. We describe, for the first time, a SYBR Green real-time RT-PCR system validated to detect all 8 EV-A71 genogroups. This tool could permit the early detection and shift in genogroup circulation and the standardization of HFMD virological diagnosis, facilitating networking of laboratories working on EV-A71 in different regions.
URI
http://hdl.handle.net/11267/2355
ISSN
1932-6203
Appears in Collections:
5.Mahosot Hospital > Journal articles
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