In vitro antimalarial drug susceptibility and pfcrt mutation among fresh Plasmodium falciparum isolates from the Lao PDR (Laos)

Title
In vitro antimalarial drug susceptibility and pfcrt mutation among fresh Plasmodium falciparum isolates from the Lao PDR (Laos)
Authors
Mayxay, M; Barends, M; Brockman, A; Jaidee, A; Nair, S; Sudimack, D; Pongvongsa, T; Phompida, S; Phetsouvanh, R; Anderson, T; White, NJ; Newton, PN
Keywords
ARTESUNATE PLUS MEFLOQUINE; SULFADOXINE-PYRIMETHAMINE; ARTEMETHER-LUMEFANTRINE; CHLOROQUINE RESISTANCE; THERAPEUTIC-EFFICACY; MOLECULAR MARKERS; WESTERN BORDER; PFMDR1 GENE; MALARIA; THAILAND
Issue Date
2007
Publisher
AM J TROP MED HYG
Citation
Am. J. Trop. Med. Hyg.;FEB;2007;76;2
Abstract
Recent drug trials in Laos have shown high levels of Plasmodium falciparum resistance to chloroquine, but there are no published data on in vitro antimalarial drug susceptibility. We used the double-site enzyme-linked pLDH immunodetection (DELI) assay to estimate the in vitro antimalarial drug susceptibility of 108 fresh P. falciparum isolates from southern Laos. The geometric mean (95% confidence interval) 500% inhibitory concentration values (nmol/L) were 152.4 (123.8-187.6) for chloroquine, 679.8 (533.8-863.0) for quinine, 45.9 (37.9-55.7) for mefloquine, 5.0 (4.4-6.4) for artesunate, 6.3 (4.5-8.9) for dihydroartemisinin, and 59.1 (46.4-75.3) for lumefantrine. The proportion of isolates defined as resistant were 65%, 40%, and 8% for chloroquine, quinine, and mefloquine, respectively. Of 53 isolates genotyped for the pfcrt T76K chloroquine-resistance mutation, 48 (91 %) were mutants. P. falciparum in Laos is multi-drug resistant; antimalarial immunity resulting from the use of ineffective chloroquine before 2005 probably contributes significantly to the therapeutic responses in clinical trials.
URI
http://hdl.handle.net/11267/2379
ISSN
0002-9637
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5.Mahosot Hospital > Journal articles
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