A Prospective Assessment of the Accuracy of Commercial IgM ELISAs in Diagnosis of Japanese Encephalitis Virus Infections in Patients with Suspected Central Nervous System Infections in Laos

Title
A Prospective Assessment of the Accuracy of Commercial IgM ELISAs in Diagnosis of Japanese Encephalitis Virus Infections in Patients with Suspected Central Nervous System Infections in Laos
Authors
Moore, CE; Blacksell, SD; Taojaikong, T; Jarman, RG; Gibbons, RV; Lee, SJ; Chansamouth, V; Thongpaseuth, S; Mayxay, M; Newton, PN
Keywords
LINKED IMMUNOSORBENT ASSAYS; IMMUNOGLOBULIN-M ANTIBODIES; CEREBROSPINAL-FLUID; HEMORRHAGIC-FEVER; CAPTURE ELISA; CHILDREN; VIETNAM; SAMPLES; DENGUE; SERUM
Issue Date
2012
Publisher
AM J TROP MED HYG
Citation
Am. J. Trop. Med. Hyg.;JUL;2012;87;1
Abstract
Japanese encephalitis virus (JEV) is a major cause of encephalitis in Asia. We estimated the diagnostic accuracy of two anti-JEV immunoglobulin M (IgM) enzyme-linked immunosorbent assays (ELISAs) (Panbio and XCyton JEVCheX) compared with a reference standard (AFRIMS JEV MAC ELISA) in a prospective study of the causes of central nervous system infections in Laos. Cerebrospinal fluid (CSF; 515 patients) and serum samples (182 patients) from those admitted to Mahosot Hospital, Vientiane, were tested. The CSF from 14.5% of acute encephalitis syndrome (AES) patients and 10.1% from those with AES and meningitis were positive for anti-JEV IgM in the reference ELISA. The sensitivities for CSF were 65.4% (95% confidence interval [CI] = 51-78) (Xcyton), 69.2% (95% CI = 55-81) (Panbio), however 96.2% (95% CI = 87-100) with Panbio Ravi criteria. Specificities were 89-100%. For admission sera from AES patients, sensitivities and specificities of the Panbio ELISA were 85.7% (95% CI = 42-100%) and 92.9% (95% CI = 83-98%), respectively.
URI
http://hdl.handle.net/11267/2399
ISSN
0002-9637
Appears in Collections:
5.Mahosot Hospital > Journal articles
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