The Diversity and Geographical Structure of Orientia tsutsugamushi Strains from Scrub Typhus Patients in Laos

Title
The Diversity and Geographical Structure of Orientia tsutsugamushi Strains from Scrub Typhus Patients in Laos
Authors
Phetsouvanh, R; Sonthayanon, P; Pukrittayakamee, S; Paris, DH; Newton, PN; Feil, EJ; Day, NPJ
Keywords
MURINE TYPHUS; TYPING DATA; RECOMBINATION; RICKETTSIA; ASSAYS; FEVER; INFECTIONS; VIENTIANE; DIAGNOSIS; GENOTYPES
Issue Date
2015
Publisher
PLOS NEGLECT TROP D
Citation
Plos Neglect. Trop. Dis.;AUG;2015;9;8
Abstract
Orientia tsutsugamushi is the causative agent of scrub typhus, a disease transmitted by Leptotrombidium mites which is responsible for a severe and under-reported public health burden throughout Southeast Asia. Here we use multilocus sequence typing (MLST) to characterize 74 clinical isolates from three geographic locations in the Lao PDR (Laos), and compare them with isolates described from Udon Thani, northeast Thailand. The data confirm high levels of diversity and recombination within the natural O. tsutsugamushi population, and a rate of mixed infection of similar to 8%. We compared the relationships and geographical structuring of the strains and populations using allele based approaches (eBURST), phylo-genetic approaches, and by calculating F-statistics (F-ST). These analyses all point towards low levels of population differentiation between isolates from Vientiane and Udon Thani, cities which straddle the Mekong River which defines the Lao/Thai border, but with a very distinct population in Salavan, southern Laos. These data highlight how land use, as well as the movement of hosts and vectors, may impact on the epidemiology of zoonotic infections.
URI
http://hdl.handle.net/11267/2423
ISSN
1935-2735
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5.Mahosot Hospital > Journal articles
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