Antibiotic resistance conferred by a class I integron and SXT constin in Vibrio cholerae O1 strains isolated in Laos

Title
Antibiotic resistance conferred by a class I integron and SXT constin in Vibrio cholerae O1 strains isolated in Laos
Authors
Iwanaga, M; Toma, C; Miyazato, T; Insisiengmay, S; Nakasone, N; Ehara, M
Keywords
CONJUGATIVE PLASMID; TYPHIMURIUM DT104; GENE CASSETTES; ELEMENT; IDENTIFICATION; MOBILIZATION; DETERMINANTS; OUTBREAK; EPIDEMIC; BENGAL
Issue Date
2004
Publisher
ANTIMICROB AGENTS CH
Citation
Antimicrob. Agents Chemother.;JUL;2004;48;7
Abstract
Changes in the drug susceptibility pattern were observed in Vibrio cholerae O1 isolated in the Lao People's Democratic Republic during 1993 to 2000. In this study, 50 V. cholerae O1 strains were selected during this period for studying the presence of class I integron and SXT constin. Twenty-four streptomycin-resistant strains out of 26 isolated before 1997 contained a class I integron harboring the aadA1 gene cassette. Twenty-four strains isolated after 1997 contained an SXT constin (a large conjugative element). Twenty of the strains were resistant to chloramphenicol, tetracycline, streptomycin, and trimethoprim-sulfamethoxazole, while four strains were susceptible to the antibiotic tested. The resistance genes included in the SXT constins were floR, tetA, strAB, and sulII, which encode resistance to chloramphenicol, tetracycline, streptomycin, and sulfamethoxazole, respectively. The antibiotic resistance gene cluster was found to be deleted in the four susceptible strains. SXTLAOS did not contain dfrA1 or dfr18, which confer resistance to trimethoprim in SXT ET and SXTMO10, respectively. A hot spot region of SXTLAOS was sequenced, and we identified two novel open reading frames showing homology to sO24 (exonuclease) and sO23 (helicase) of the genomic island associated with the multidrug resistance region of Salmonella enterica serovar Typhimurium DT104. Analysis of SXTLAOS showed that there is a continuous flux of genes among V. cholerae SXT constins which should be carefully monitored.
URI
http://hdl.handle.net/11267/2598
ISSN
0066-4804
Appears in Collections:
2.Ministry of Health > Journal articles
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