The incidence of Escherichia coli having pathogenic genes for diarrhea: A study in the people's democratic Republic of Lao

Title
The incidence of Escherichia coli having pathogenic genes for diarrhea: A study in the people's democratic Republic of Lao
Authors
Phantouamath, B; Sithivong, N; Insisiengmay, S; Higa, N; Toma, C; Nakasone, N; Iwanaga, M
Keywords
POLYMERASE-CHAIN-REACTION; MULTIPLEX PCR; IDENTIFICATION; STRAINS; AMPLIFICATION; ASSAY
Issue Date
2003
Publisher
JPN J INFECT DIS
Citation
Jpn. J. Infect. Dis.;JUN;2003;56;3
Abstract
The incidence of Escherichia coli having pathogenic genes for diarrhea was studied in Laos in 2002. A total of 525 E.coli strains from 278 patients (basically, two E. coli isolates from each patient) were examined by PCR to detect the known pathogenic genes (stx, eae, elt, est, ipaH, and aggR). These genes were detected in 23 strains from 16 patients (16/278: 5.8%). In 10 cases of the 16, one of the two isolates from each individual was negative for the gene, and in the other six cases, both isolates had the gene (same gene in four cases). E. coli having eae but no stx (enteropathogenic E. coli [EPEC]) was found in two cases out of 278 (0.7%). Nevertheless, Class I classical EPEC (serogroup-based) was found in 77 cases (28%). Enterotoxigenic E. coli, enteroaggregative E. coli, and enterohemorrhagic E. coli were found in 9, 4, and I cases, respectively. Enteroinvasive E. coli was not detected. This study suggested that the incidence of diarrhea due to E. coli is not as high as has been previously thought.
URI
http://hdl.handle.net/11267/2607
ISSN
1344-6304
Appears in Collections:
2.Ministry of Health > Journal articles
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