Sensitive and specific enzyme-linked immunosorbent assay for the diagnosis of Wuchereria bancrofti infection in urine samples

Title
Sensitive and specific enzyme-linked immunosorbent assay for the diagnosis of Wuchereria bancrofti infection in urine samples
Authors
Itoh, M; Weerasooriya, MV; Qiu, XG; Gunawardena, NK; Anantaphruti, MT; Tesana, S; Rattanaxay, P; Fujimaki, Y; Kimura, E
Keywords
LYMPHATIC FILARIASIS; MONOCLONAL-ANTIBODY; ANTIGENS; IGG4; DIETHYLCARBAMAZINE; SCHISTOSOMIASIS; INDIVIDUALS; PREVALENCE; RESPONSES; DISEASE
Issue Date
2001
Publisher
AM J TROP MED HYG
Citation
Am. J. Trop. Med. Hyg.;OCT;2001;65;4
Abstract
We developed an enzyme-linked immunosorbent assay (ELISA) that detects filaria-specific immunoglobulin G4 antibodies in unconcentrated urine. The ELISA was positive in 87 of 91 (95.6%) urine samples collected from people with Wuchereria bancrofti microfilariae, antigen, or both. Of 298 urine samples collected in Thailand, Lao People's Democratic Republic, and Japan, where no human filariasis is known, 295 (99.0%,) were negative by ELISA. Various intestinal nematode and fluke infections did not interfere with the ELISA. Urine samples with sodium azide could be kept at 37 degreesC for 4 weeks, and the time of urine collection did not influence ELISA results. This ELISA can be used to identify endemic foci of filariasis.
URI
http://hdl.handle.net/11267/2613
ISSN
0002-9637
Appears in Collections:
2.Ministry of Health > Journal articles
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